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Delatylus andersoni n. gen., n. sp. (Nematoda: Neotylenchidae) Isolated from White Pine (Pinus monticola) Lumber from USA and Intercepted in Ningbo, China Cover

Delatylus andersoni n. gen., n. sp. (Nematoda: Neotylenchidae) Isolated from White Pine (Pinus monticola) Lumber from USA and Intercepted in Ningbo, China

Open Access
|May 2018

Figures & Tables

Figure 1

Line drawings of female Delatylus andersoni n. gen., n. sp: (A) head and pharynx, (B) lip region and stylet, (C) deirids, (D) vulva and the anus viewed ventrally, (E) vulva and the tail viewed laterally, (F) oviduct and quadricolumella, (G) oocytes.
Line drawings of female Delatylus andersoni n. gen., n. sp: (A) head and pharynx, (B) lip region and stylet, (C) deirids, (D) vulva and the anus viewed ventrally, (E) vulva and the tail viewed laterally, (F) oviduct and quadricolumella, (G) oocytes.

Figure 2

Micrographs of females of Delatylus andersoni n. gen., n. sp: (A) entire body; (B) head region, (C) pharynx (arrow showing the position of excretory pore and hemizonid), (D) pharynx with anterior part of the gonad, (E) posterior part of the female body, (F, G) female tail (arrow showing the position of vulva and anus), H: lateral lines. (scale bars = 10 μm).
Micrographs of females of Delatylus andersoni n. gen., n. sp: (A) entire body; (B) head region, (C) pharynx (arrow showing the position of excretory pore and hemizonid), (D) pharynx with anterior part of the gonad, (E) posterior part of the female body, (F, G) female tail (arrow showing the position of vulva and anus), H: lateral lines. (scale bars = 10 μm).

Figure 3

Micrographs of valve at the pharyngeal–intestinal junction of females of Delatylus andersoni n. gen., n. sp.
Micrographs of valve at the pharyngeal–intestinal junction of females of Delatylus andersoni n. gen., n. sp.

Figure 4

SEM of Delatylus andersoni n. gen., n. sp. (A, B) En face view of head and lip (aa arrows point to the amphid aperture; oa arrows point to the oral aperture; op arrows point to outer labial papillae; (C) vulva, anus, and tail; (D) lateral fields.
SEM of Delatylus andersoni n. gen., n. sp. (A, B) En face view of head and lip (aa arrows point to the amphid aperture; oa arrows point to the oral aperture; op arrows point to outer labial papillae; (C) vulva, anus, and tail; (D) lateral fields.

Figure 5

Phylogeny of Delatylus andersoni n. gen., n. sp. and closely related inferred from partial 18 S rRNA gene sequences by Bayesian analysis. Phylogeny was inferred under a GTR+I+G model (−lnL = 6333.834; AIC = 12687.668; freqA = 0.2384; freqC = 0.2036; freqG = 0.267; freqT = 0.2911; R(a) = 1.1233; R(b) = 3.698; R(c) = 2.4739; R(d) = 0.7402; R(e) = 5.1375; R(f) = 1; Pinva = 0.6096; Shape = 0.8015). Posterior probability values exceeding 50% are given on appropriate clades.
Phylogeny of Delatylus andersoni n. gen., n. sp. and closely related inferred from partial 18 S rRNA gene sequences by Bayesian analysis. Phylogeny was inferred under a GTR+I+G model (−lnL = 6333.834; AIC = 12687.668; freqA = 0.2384; freqC = 0.2036; freqG = 0.267; freqT = 0.2911; R(a) = 1.1233; R(b) = 3.698; R(c) = 2.4739; R(d) = 0.7402; R(e) = 5.1375; R(f) = 1; Pinva = 0.6096; Shape = 0.8015). Posterior probability values exceeding 50% are given on appropriate clades.

Figure 6

Phylogeny of Delatylus andersoni n. gen., n. sp. and closely related inferred from 28 S D2/D3 rRNA gene sequences by Bayesian analysis. Phylogeny was inferred under a TrN+G model (−lnL = 2644.97; AIC = 5301.9399; freqA = 0.2103; freqC = 0.1993; freqG = 0.3249; freqT = 0.2655; R(a) = 1; R(b) = 3.0953; R(c) = 1; R(d) = 1; R(e) = 7.2585; R(f) = 1; Pinva = 0; Shape = 0.3542). Posterior probability values exceeding 50% are given on appropriate clades.
Phylogeny of Delatylus andersoni n. gen., n. sp. and closely related inferred from 28 S D2/D3 rRNA gene sequences by Bayesian analysis. Phylogeny was inferred under a TrN+G model (−lnL = 2644.97; AIC = 5301.9399; freqA = 0.2103; freqC = 0.1993; freqG = 0.3249; freqT = 0.2655; R(a) = 1; R(b) = 3.0953; R(c) = 1; R(d) = 1; R(e) = 7.2585; R(f) = 1; Pinva = 0; Shape = 0.3542). Posterior probability values exceeding 50% are given on appropriate clades.

Morphometrics of Delatylus andersoni n_ gen_, n_ sp_ All measurements are in μm and in the form: mean ± s_d (range)_

Measurements
HolotypePopulations
Characters 9095 (paratype)480484
n1192020
L760756.1 ± 113.7 (526.8–943.9)613.8 ± 96.3 (439.5–829.6)760.0 ± 73.8 (636.6–907.2)
a27.128.8 ± 2.4 (24.9–33.5)23.7 ± 2.7 (18.3–27.9)22.0 ± 2.7 (17.8–27.1)
b10.811.1 ± 1.7 (7.5–13.7)9.6 ± 1.6 (7.0–13.8)11.6 ± 1.4 (9.3–13.8)
b'5.65.9 ± 0.8 (4.1–7.1)5.8 ± 0.9 (4.3–8.3)6.6 ± 0.6 (5.4–7.4)
c13.814.3 ± 2.1 (10.3–17.9)13.7 ± 1.2 (11.3–15.5)15.0 ± 1.7 (11.8–18.9)
c'3.23.4 ± 0.2 (2.9–3.8)3.2 ± 0.2 (2.8–3.8)3.1 ± 0.4 (2.6–4.1)
V8989.9 ± 1.5 (85.6–91.8)88.8 ± 0.8 (87.3–90.3)89.5 ± 1.1 (87.3–91.8)
Stylet length9.08.8 ± 0.3 (8.5–10.5)8.8 ± 0.6 (7.8–9.7)9.2 ± 0.7 (7.3–10.1)
Max. body diam.2826.4 ± 4.6 (18.4–33.9)26.4 ± 6.2 (20.1–40.5)35.1 ± 6.3 (23.5–49.8)
Gonad length595526.0 ± 10 (317.7–677.9)461.1 ± 91.5 (261.6–685.5)603.1 ± 74.7 (494.5–753.8)
Lip height22.1 ± 0.2 (1.8–2.6)2.2 ± 0.2 (1.9–2.8)2.3 ± 0.3 (2.0–2.9)
Lip width88.3 ± 0.5 (7.1–9.1)7.4 ± 0.6 (6.6–8.7)8.0 ± 0.7 (6.4–8.9)
Anterior end to nerve ring6561.1 ± 3.8 (55.1–70.8)55.9 ± 3.2 (50.8–64.7)58.6 ± 4.5 (50.2–66.2)
Anterior end to pharyngo-intestinal junction7068.3 ± 3.3 (62.5–75.1)63.9 ± 3.8 (58.1–72.3)65.7 ± 4.5 (55.8–73.0)
Anterior end to the hemizonid110104.0 ± 8.2 (91.3–122.5)95.7 ± 13.1 (79.8–128.6)107.0 ± 7.5 (90.7–119.9)
Distance from the nerve ring to the hemizonid5043.0 ± 6.4 (31.6–57.2)36.8 ± 13.0 (23.4–69.0)48.4 ± 9.2 (29.1–61.3)
Anterior end to the excretory pore113107.1 ± 7.6 (95.6–124.5)92.6 ± 13.0 (76.7–125.4)103.0 ± 7.4 (86.0–114.6)
Distance from the nerve ring to the excretory pore5146.0 ± 5.6 (37.8–59.2)36.8 ± 13.0 (23.4–69.0)44.4 ± 9.2 (24.4–58.1)
Anterior end to the end of pharyngeal glands135128.3 ± 7.7 (113.2–146.6)106.4 ± 10.2 (90.5–127.4)115.0 ± 5.3 (105.7–125.3)
Distance from vulva to anus2622.3 ± 3.3 (16.0–28.3)23.3 ± 3.6 (18.8–31.3)27.8 ± 3.0 (21.5–33.3)
Anal body width (ABW)1715.6 ± 2.0 (11.3–19.8)14.1 ± 1.6 (11.9–16.9)16.5 ± 2.0 (12.5–21.0)
Distance from vulva to tail terminus8175.3 ± 5.4 (60.0–83.9)67.9 ± 7.2 (55.2–84.9)78.8 ± 5.2 (72.8–89.3)
Tail length5553.1 ± 4.6 (39.3–58.9)44.7 ± 4.8 (35.5–53.6)51.0 ± 4.0 (45.8–59.6)
Ratio of vulva–anus/tail length0.470.5 ± 0.1 (0.4–0.6)0.5 ± 0.2 (0.4–0.7)0.5 ± 0.1 (0.4–0.5)
DOI: https://doi.org/10.21307/jofnem-2018-013 | Journal eISSN: 2640-396X | Journal ISSN: 0022-300X
Language: English
Page range: 69 - 76
Accepted on: Jul 7, 2017
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Published on: May 31, 2018
In partnership with: Paradigm Publishing Services
Publication frequency: 1 issue per year

© 2018 Qing Yu, Maria Munawar, Jianfeng Gu, Weimin Ye, published by Society of Nematologists, Inc.
This work is licensed under the Creative Commons Attribution 4.0 License.