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Description of Aphelenchoides giblindavisi n. sp. (Nematoda: Aphelenchoididae), and Proposal for a New Combination Cover

Description of Aphelenchoides giblindavisi n. sp. (Nematoda: Aphelenchoididae), and Proposal for a New Combination

Open Access
|Oct 2018

Figures & Tables

Fig. 1

Line drawings of Aphelenchoides giblindavisi n. sp. (A, B) Entire body of female and male. (C) Anterior end. (D, E) Pharynx. (F) Part of female reproductive system. (G) Tail end. (H, I) Tail of female. (J) Posterior body region of male.
Line drawings of Aphelenchoides giblindavisi n. sp. (A, B) Entire body of female and male. (C) Anterior end. (D, E) Pharynx. (F) Part of female reproductive system. (G) Tail end. (H, I) Tail of female. (J) Posterior body region of male.

Fig. 2

Light microphotographs of Aphelenchoides giblindavisi n. sp. (A–C) Anterior region. (D) Part of female reproductive system (junction of oviduct and spermatheca). (E) Spicule. (F) Vulva region and postvulval uterine sac. (G, H) Posterior body region of female. (I) Tail end of male (All scale bars = 10 μm).
Light microphotographs of Aphelenchoides giblindavisi n. sp. (A–C) Anterior region. (D) Part of female reproductive system (junction of oviduct and spermatheca). (E) Spicule. (F) Vulva region and postvulval uterine sac. (G, H) Posterior body region of female. (I) Tail end of male (All scale bars = 10 μm).

Fig. 3

Scanning electron microphotographs of Aphelenchoides giblindavisi n. sp. (A) Details of anterior region. (B, C) Lip region. (D, E) Lateral lines in different parts of mid-body. (F) Details of tail end. (G) Female posterior body region. (H, I) Male caudal region.
Scanning electron microphotographs of Aphelenchoides giblindavisi n. sp. (A) Details of anterior region. (B, C) Lip region. (D, E) Lateral lines in different parts of mid-body. (F) Details of tail end. (G) Female posterior body region. (H, I) Male caudal region.

Fig. 4

Scanning electron microphotograph of Aphelenchoides giblindavisi n. sp. Details of lip region. Abbreviations: il9–inner lip papillae, aa–amphidial openings, olp–outer labial papillae, cp–cephalic papillae, ld–labial disc.
Scanning electron microphotograph of Aphelenchoides giblindavisi n. sp. Details of lip region. Abbreviations: il9–inner lip papillae, aa–amphidial openings, olp–outer labial papillae, cp–cephalic papillae, ld–labial disc.

Fig. 5

Line drawings of Iranian population of Robustodorus helicus n. comb. (A) Anterior end. (B, C) Female total body. (D, F) Pharynx. (E) Stylet. (G, H) Posterior body region.
Line drawings of Iranian population of Robustodorus helicus n. comb. (A) Anterior end. (B, C) Female total body. (D, F) Pharynx. (E) Stylet. (G, H) Posterior body region.

Fig. 6

Light microphotographs of fresh females of Iranian population of Robustodorus helicus n. comb. (A) Anterior region. (B, H) Part of pharynx. (C) Anterior end. (D) Excretory pore. (E) Metacorpus. (F) Tail. (G) Embryo inside the mature egg inside the body of female. (All scale bars = 10 μm).
Light microphotographs of fresh females of Iranian population of Robustodorus helicus n. comb. (A) Anterior region. (B, H) Part of pharynx. (C) Anterior end. (D) Excretory pore. (E) Metacorpus. (F) Tail. (G) Embryo inside the mature egg inside the body of female. (All scale bars = 10 μm).

Fig. 7

Light microphotographs of Iranian population of Robustodorus helicus n. comb. (A) Three tulip-shaped lips in lateral view. (B, C) Three lines in the lateral field forming two bands. (D, E) Postvulval uterine sac. (F) Anterior region. (All scale bars = 10 μm).
Light microphotographs of Iranian population of Robustodorus helicus n. comb. (A) Three tulip-shaped lips in lateral view. (B, C) Three lines in the lateral field forming two bands. (D, E) Postvulval uterine sac. (F) Anterior region. (All scale bars = 10 μm).

Fig. 8

Bayesian tree inferred under the GTR + G + I model using SSU rDNA sequences of Aphelenchoides giblindavisi n. sp. and Robustodorus helicus n. comb. Posterior probability and bootstrap values exceeding 50% are given on appropriate clades in the form Bayesian posterior probability/maximum likelihood bootstrap value (BPP/BS). The new sequences are in bold font.
Bayesian tree inferred under the GTR + G + I model using SSU rDNA sequences of Aphelenchoides giblindavisi n. sp. and Robustodorus helicus n. comb. Posterior probability and bootstrap values exceeding 50% are given on appropriate clades in the form Bayesian posterior probability/maximum likelihood bootstrap value (BPP/BS). The new sequences are in bold font.

Fig. 9

Bayesian tree inferred under the GTR + G + I model using partial LSU rDNA sequence of Aphelenchoides giblindavisi n. sp. and Robustodorus helicus n. comb. Posterior probability and bootstrap values exceeding 50% are given on appropriate clades in the form Bayesian posterior probability/maximum likelihood bootstrap value (BPP/BS). The new sequences are in bold font. The asterisk on Laimaphelenchus hyrcanus Miraeiz et al. (2015) refers to the possible synonymy of the species with L. belgradiensis Oro, 2015 as discussed by Pedram et al. (2018a, 2018b).
Bayesian tree inferred under the GTR + G + I model using partial LSU rDNA sequence of Aphelenchoides giblindavisi n. sp. and Robustodorus helicus n. comb. Posterior probability and bootstrap values exceeding 50% are given on appropriate clades in the form Bayesian posterior probability/maximum likelihood bootstrap value (BPP/BS). The new sequences are in bold font. The asterisk on Laimaphelenchus hyrcanus Miraeiz et al. (2015) refers to the possible synonymy of the species with L. belgradiensis Oro, 2015 as discussed by Pedram et al. (2018a, 2018b).

Morphometrics of Aphelenchoides giblindavisi n_ sp_ from Iran_ All measurements are in μm and in the form: mean ± S_D_ (range)_

FemaleMale
CharacterHolotypeParatypesParatypes
n1212
L653671 ± 72.4 (546–795)632 ± 41.4 (523–679)
a39.634.9 ± 2.2 (31.4–39.6)37.4 ± 2.2 (32.4–41.0)
b8.68.9 ± 0.8 (7.4–10.1)8.7 ± 0.4 (8.0–9.4)
b′4.54.5 ± 0.4 (3.9–5.0)4.5 ± 0.2 (4.2–5.0)
c18.718.9 ± 1.0 (17.2–20.4)18.9 ± 1.7 (15.8–21.5)
c′3.53.4 ± 0.2 (3.1–3.7)2.8 ± 0.2 (2.6–3.1)
V or T70.170.6 ± 1.6 (68.8–73.5)52.8 ± 3.4 (47.4–59.2)
Head height2.52.5 ± 0.0 (2.0–2.5)2.5 ± 0.0 (2.0–2.5)
Head diam.5.55.5 ± 0.4 (5–6)5.5 ± 0.3 (5–6)
Stylet10.510.5 ± 0.5 (10–11)10.0 ± 0.4 (10–11)
Stylet conus4.54.5 ± 0.2 (4.0–4.5)4.0 ± 0.2 (3.5–4.5)
m42.938.9 ± 2.3 (36.4–42.9)38.4 ± 2.1 (35–40)
Median bulb5858.0 ± 3.2 (54–65)56.0 ± 1.4 (54–58)
Excretory pore6969.0 ± 6.2 (60–84)66.5 ± 1.8 (64–69)
Hemizonid8089.0 ± 10.6 (77–110)81.0 ± 2.6 (78–85)
Pharynx7675.5 ± 2.8 (71–81)72.0 ± 3.4 (65–78)
Nerve ring7575.0 ± 2.8 (71–81)73.0 ± 2.3 (70–78)
Median bulb length13.514 ± 1 (12–15)13.0 ± 0.9 (11–14)
Median bulb diam.10.511.0 ± 1.1 (9.0–12.5)10.0 ± 0.9 (8.0–11.5)
Median bulb length/diam.1.31.3 ± 0.1 (1.1–1.4)1.3 ± 0.1 (1.2–1.6)
Pharyngeal overlapping7073.0 ± 6.7 (63–87)67.0 ± 6.4 (60–80)
Maximum body diam.16.519.0 ± 2.4 (15–23)17.0 ± 1.4 (14–19)
Vulval body diam. (VBD)1618.0 ± 1.8 (14.5–20.0)
Body diam. at median bulb13.514.0 ± 1.4 (12.0–15.5)13.0 ± 0.8 (11–14)
Postvulval uterine sac (PUS)6562.4 ± 6.0 (52–69)
PUS/VBD4.13.5 ± 0.4 (2.7–4.1)
Vulva–anus160161 ± 21.6 (114–190)
Ovary or testis length295257 ± 58.7 (183–356)334 ± 35 (248–394)
Anal (cloacal) body width1010 ± 1 (9.0–12.5)12.0 ± 0.6 (10.5–13.0)
Tail length3535.0 ± 2.5 (30–39)34.0 ± 1.8 (31–37)
Spicules length (arc line)17.0 ± 0.7 (16–18)
Capitulum7.0 ± 0.5 (6–8)

Morphometrics of Iranian population of Robustodorus helicus n_ comb_ All measurements are in μm and in the form: mean ± S_D_ (range)_

Females
n15
L464 ± 37.3 (395–547)
a29.2 ± 1.8 (26.7–33.5)
b7.8 ± 0.6 (6.7–8.8)
b′4.6 ± 0.4 (3.8–5.4)
c19.7 ± 1.7 (16.8–22.8)
c′2.8 ± 0.3 (2.4–3.1)
V69.3 ± 1.6 (64.4–71.1)
Head height2.0 ± 0.3 (2.0–2.5)
Head diam.5.0 ± 0.4 (4.5–5.5)
Stylet10.0 ± 0.4 (10–11)
Stylet conus4.0 ± 0.3 (4.0–4.5)
m41.3 ± 2.8 (36.4–45.0)
Median bulb47.5 ± 2.9 (39–51)
Excretory pore53.0 ± 4.8 (46–62)
Hemizonid63.0 ± 5.6 (53–69)
Pharynx60 ± 4 (49–65)
Nerve ring58 ± 4 (45–62)
Median bulb length11.5 ± 0.9 (10.0–13.5)
Median bulb diam.10.0 ± 0.8 (8–11)
Median bulb length/diam.1.2 ± 0.1 (1.1–1.3)
Pharyngeal overlapping40 ± 5 (31–49)
Maximum body diam.16.0 ± 1.6 (13–20)
Vulval body diam. (VBD)15.0 ± 1.4 (12–19)
Body diam. at median bulb13.0 ±1.1 (11.5–15.0)
Postvulval uterine sac (PUS)38 ± 8 (28–55)
PUS/VBD2.5 ± 0.5 (2.0–3.7)
Vulva–anus119 ± 13 (101–147)
Ovary length161 ± 20 (130–193)
Anal body diam.8.0 ± 0.9 (7–10)
Tail length24.0 ± 1.4 (21–26)
DOI: https://doi.org/10.21307/jofnem-2018-035 | Journal eISSN: 2640-396X | Journal ISSN: 0022-300X
Language: English
Page range: 437 - 452
Published on: Oct 17, 2018
In partnership with: Paradigm Publishing Services
Publication frequency: 1 issue per year

© 2018 Farzad Aliramaji, Ebrahim Pourjam, Sergio Álvarez-Ortega, Farahnaz Jahanshahi Afshar, Majid Pedram, published by Society of Nematologists, Inc.
This work is licensed under the Creative Commons Attribution 4.0 License.