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Enhanced entomopathogenic nematode yield and fitness via addition of pulverized insect powder to solid media Cover

Enhanced entomopathogenic nematode yield and fitness via addition of pulverized insect powder to solid media

Journal of Nematology
Volume 50 (2018): Issue 4 (January 2018)
By: Shiyu Zhen,  Yang Li,  Yanli Hou,  Xinghui Gu,  Limeng Zhang,  Weibin Ruan and  David Shapiro-Ilan  
Open Access
|Dec 2018

Figures & Tables

Figure 1

Effect of insect powder and dose level on infective juveniles yield (h) in solid culture media. G: Galleria mellonella; T: Tenebrio molitor; L: Lucillia sericata; G+T:1:1 mixed powder of G. mellonella and T. molitor; G+L, 1:1 mixed powder of G. mellonella and L. sericata; T+L: 1:1 mixed powder of T. molitor and L. sericata. L, M, H: three dose levels of insect powder 1, 3, and 5% (m/m), respectively. Bars with different letters represent significantly different means (SE) at p ⩽ 0.05 among all treatments via the post hoc Duncan’s test.
Effect of insect powder and dose level on infective juveniles yield (h) in solid culture media. G: Galleria mellonella; T: Tenebrio molitor; L: Lucillia sericata; G+T:1:1 mixed powder of G. mellonella and T. molitor; G+L, 1:1 mixed powder of G. mellonella and L. sericata; T+L: 1:1 mixed powder of T. molitor and L. sericata. L, M, H: three dose levels of insect powder 1, 3, and 5% (m/m), respectively. Bars with different letters represent significantly different means (SE) at p ⩽ 0.05 among all treatments via the post hoc Duncan’s test.

Figure 2

Effect of insect powder and dose level on the time-to-death (h) of Spodoptera litura larvae in two trials. Each larva was exposed to 100 Steinernema feltiae infective juveniles. Five larvae in five wells were regarded as a replication of each treatment. G: Galleria mellonella; T: Tenebrio molitor; L: Lucillia sericata; G+T:1:1 mixed powder of G. mellonella and T. molitor; G+L, 1:1 mixed powder of G. mellonella and L. sericata; T+L: 1:1 mixed powder of T. molitor and L. sericata. : L, M, H: three dose levels of insect powder 1, 3, and 5% (m/m), respectively. Bars with different letters represent significantly different means (SE) at p ⩽ 0.05 among all treatments via the post hoc Duncan’s test.
Effect of insect powder and dose level on the time-to-death (h) of Spodoptera litura larvae in two trials. Each larva was exposed to 100 Steinernema feltiae infective juveniles. Five larvae in five wells were regarded as a replication of each treatment. G: Galleria mellonella; T: Tenebrio molitor; L: Lucillia sericata; G+T:1:1 mixed powder of G. mellonella and T. molitor; G+L, 1:1 mixed powder of G. mellonella and L. sericata; T+L: 1:1 mixed powder of T. molitor and L. sericata. : L, M, H: three dose levels of insect powder 1, 3, and 5% (m/m), respectively. Bars with different letters represent significantly different means (SE) at p ⩽ 0.05 among all treatments via the post hoc Duncan’s test.

Figure 3

Effect of insect powder and dose level on the progeny yield (h) of Spodoptera litura larvae in two trials. Each larva was exposed to 100 Steinernema feltiae infective juveniles. Five larvae in five wells were regarded as a replication of each treatment. G: Galleria mellonella; T: Tenebrio molitor; L: Lucillia sericata; G+T:1:1 mixed powder of G. mellonella and T. molitor; G+L, 1:1 mixed powder of G. mellonella and L. sericata; T+L: 1:1 mixed powder of T. molitor and L. sericata. L, M, H: three dose levels of insect powder 1, 3, and 5% (m/m), respectively. Bars with different letters represent significantly different means (SE) at p ⩽ 0.05 among all treatments via the post hoc Duncan’s test.
Effect of insect powder and dose level on the progeny yield (h) of Spodoptera litura larvae in two trials. Each larva was exposed to 100 Steinernema feltiae infective juveniles. Five larvae in five wells were regarded as a replication of each treatment. G: Galleria mellonella; T: Tenebrio molitor; L: Lucillia sericata; G+T:1:1 mixed powder of G. mellonella and T. molitor; G+L, 1:1 mixed powder of G. mellonella and L. sericata; T+L: 1:1 mixed powder of T. molitor and L. sericata. L, M, H: three dose levels of insect powder 1, 3, and 5% (m/m), respectively. Bars with different letters represent significantly different means (SE) at p ⩽ 0.05 among all treatments via the post hoc Duncan’s test.

Figure S1

Effect of insect powder and dose level on the initial emergence time of infective juveniles from Spodoptera litura larvae in two trials. Each larva was exposed to 100 Steinernema feltiae infective juveniles. Five larvae in five wells were regarded as a replication of each treatment. G: Galleria mellonella; T: Tenebrio molitor; L: Lucillia sericata; G+T:1:1 mixed powder of G. mellonella and T. molitor; G+L, 1:1 mixed powder of G. mellonella and L. sericata; T+L: 1:1 mixed powder of T. molitor and L. sericata. L, M, H: three dose levels of insect powder 1, 3, and 5% (m/m), respectively. Bars with different letters represent significantly different means (SE) at p ⩽ 0.05 among all treatments via the post hoc Duncan’s test.
Effect of insect powder and dose level on the initial emergence time of infective juveniles from Spodoptera litura larvae in two trials. Each larva was exposed to 100 Steinernema feltiae infective juveniles. Five larvae in five wells were regarded as a replication of each treatment. G: Galleria mellonella; T: Tenebrio molitor; L: Lucillia sericata; G+T:1:1 mixed powder of G. mellonella and T. molitor; G+L, 1:1 mixed powder of G. mellonella and L. sericata; T+L: 1:1 mixed powder of T. molitor and L. sericata. L, M, H: three dose levels of insect powder 1, 3, and 5% (m/m), respectively. Bars with different letters represent significantly different means (SE) at p ⩽ 0.05 among all treatments via the post hoc Duncan’s test.
DOI: https://doi.org/10.21307/jofnem-2018-050 | Journal eISSN: 2640-396X | Journal ISSN: 0022-300X
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Language: English
Page range: 495 - 506
Published on: Dec 3, 2018
Published by: Society of Nematologists, Inc.
In partnership with: Paradigm Publishing Services
Publication frequency: 1 issue per year
Keywords:
EPN,
Soil culture,
Mortality time
Related subjects:
Life sciences,
Life sciences, other

© 2018 Shiyu Zhen, Yang Li, Yanli Hou, Xinghui Gu, Limeng Zhang, Weibin Ruan, David Shapiro-Ilan, published by Society of Nematologists, Inc.
This work is licensed under the Creative Commons Attribution 4.0 License.

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