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Efficacy of medicinal plants from Algeria on cell proliferation: Involvement of caspases 3/7 and cell cycle disorder Cover

Efficacy of medicinal plants from Algeria on cell proliferation: Involvement of caspases 3/7 and cell cycle disorder

Postępy Higieny i Medycyny Doświadczalnej
Volume 79 (2025): Issue 1 (January 2025)
By: Leila Gadouche,  Dagmara Klopotowska and  Joanna Wietrzyk  
Open Access
|Mar 2025

Figures & Tables

Figure 1.

Caspase-3/7 activity after 72 h of HepG2 cells treatment with extracts at 200 µg/ml concentration. Concentrations of cytostatics: Cisplatin – 1 µg/ml, Doxorubicin – 10 µM, and Camptothecin – 0,5 µg/ml. Doxorubicin and Camptothecin were added 24 h before Caspase-3/7 assay. The normality of the data distribution was performed using the Shapiro-Wilk test. One-way ANOVA tests with Dunnett’s multiple comparisons test were performed. **** - p< 0,0001 vs. ctrl. The number of observations for ctrl and Aristolochia baetica – 7, Lavendula dentata – 6, Cisplatin – 5, Doxorubicin, and Camptothecin – 3.
Caspase-3/7 activity after 72 h of HepG2 cells treatment with extracts at 200 µg/ml concentration. Concentrations of cytostatics: Cisplatin – 1 µg/ml, Doxorubicin – 10 µM, and Camptothecin – 0,5 µg/ml. Doxorubicin and Camptothecin were added 24 h before Caspase-3/7 assay. The normality of the data distribution was performed using the Shapiro-Wilk test. One-way ANOVA tests with Dunnett’s multiple comparisons test were performed. **** - p< 0,0001 vs. ctrl. The number of observations for ctrl and Aristolochia baetica – 7, Lavendula dentata – 6, Cisplatin – 5, Doxorubicin, and Camptothecin – 3.

Figure 2.

The cell cycle analysis for extracts at 1000 µg/ml after 72 hours of treatment of MV4-11 cells. Concentrations of cisplatin – 0.4 µg/ml. The number of observations for ctrl – 5, Aristolochia baetica and Lavendula dentata – 4, Cisplatin – 6. Two-way ANOVA and Dunnett’s multiple comparisons test was used for cell cycle analysis. *- p < 0.05, **- p < 0.01, ***- p < 0.001, ****-p < 0.0001 vs. ctrl
The cell cycle analysis for extracts at 1000 µg/ml after 72 hours of treatment of MV4-11 cells. Concentrations of cisplatin – 0.4 µg/ml. The number of observations for ctrl – 5, Aristolochia baetica and Lavendula dentata – 4, Cisplatin – 6. Two-way ANOVA and Dunnett’s multiple comparisons test was used for cell cycle analysis. *- p < 0.05, **- p < 0.01, ***- p < 0.001, ****-p < 0.0001 vs. ctrl

Figure 3.

The cell cycle analysis for extracts at 100 µg/ml after 72 hours of treatment of MV4-11 cells. Concentrations of cisplatin – 0.4 µg/ml. The number of observations for ctrl – 5, Aristolochia baetica and Lavendula dentata – 4, Cisplatin – 6. Two-way ANOVA and Dunnett’s multiple comparisons test was used for cell cycle analysis. *- p < 0.05, **- p < 0.01, ****-p < 0.0001 vs. ctrl.
The cell cycle analysis for extracts at 100 µg/ml after 72 hours of treatment of MV4-11 cells. Concentrations of cisplatin – 0.4 µg/ml. The number of observations for ctrl – 5, Aristolochia baetica and Lavendula dentata – 4, Cisplatin – 6. Two-way ANOVA and Dunnett’s multiple comparisons test was used for cell cycle analysis. *- p < 0.05, **- p < 0.01, ****-p < 0.0001 vs. ctrl.

The antiproliferative activity of tested extracts presented as the mean IC50 value ± standard deviation (SD)_ The extracts were dissolved in culture medium

A-498HepG2PLC/PRF/5MDA-MB-231MV4-11BALB/3T3
IC50 (mean ± SD) [μg/mL]
Aristolochia baetica430.20±138.23340.94±143.0722.25±103.98241.21±73.38277.08±87.26387.69±142.75
Cistus lanifedus528.65±277.15759.24±200.31n.a.403.77±157.14n.t.604.93±315.09
Crataegus Monogyna (Leaves)n.a.n.a.n.a.598.15±45.96n.t.n.a.
Crataegus Monogyna (Fruit)n.a.n.a.n.a.84.99±11.11*n.t.n.a.
Erica arborea347.27±106.93481.40±148.33n.a.450.48±158.56n.a.288.68±11.00
Rhamnus alaternusn.a.729.25±205.32n.a.n.a.n.t.n.a.
Lavendula dentata375.60±87.05317.38±25.75354.15±13.99277.91±24.73684.34±192.55226.85±25.63
Cisplatin2.64+±0.781.32±0.941.64±0.293.11±0.480.39±0.0470.50±0.32

Fold mean relative caspase-3/7 activity in comparison to the untreated control ± SD in HepG2 cells

Mean relative caspase-3/7 activity ± SD
Aristolochia baetica (200μg/ml)0.680±0.134
Lavendula dentata (200μg/ml)0.795±0.120
Cisplatin (1μg/ml)1.425±0.067
Doxorubicin (10μM)4.890±1.096
Camptothecin (0,5μg/ml)8.975±0.028
DOI: https://doi.org/10.2478/ahem-2025-0003 | Journal eISSN: 1732-2693
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Language: English
Page range: 16 - 24
Submitted on: Sep 18, 2024
|
Accepted on: Jan 15, 2025
|
Published on: Mar 19, 2025
Published by: Hirszfeld Institute of Immunology and Experimental Therapy
In partnership with: Paradigm Publishing Services
Publication frequency: 1 issue per year
Keywords:
Aristolochia baetica,
Lavandula dentata, caspases 3/7,
antiproliferative activity,
cell cycle
Related subjects:
Life sciences,
Molecular biology,
Microbiology and virology,
Medicine,
Basic medical science,
Immunology

© 2025 Leila Gadouche, Dagmara Klopotowska, Joanna Wietrzyk, published by Hirszfeld Institute of Immunology and Experimental Therapy
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

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