A tendency to maintain a stable level of sulfane sulfur under various environmental or culture conditions Cover

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A tendency to maintain a stable level of sulfane sulfur under various environmental or culture conditions

Postępy Higieny i Medycyny Doświadczalnej

Volume 79 (2025): Issue 1 (January 2025)

By: Halina Jurkowska , Leszek Rydz , Marta Kaczor-Kamińska and Maria Wróbel

Open Access

|Dec 2025

Figures & Tables

Synthesis and transformation of sulfane sulfur in biological systemsAbbreviations: S - sulfane sulfur atom (marked in red); (1) - S-sulfuration reaction; (2) - S-nitrosylation reaction; CBS - cystathionine β-synthase; CTH - cystathionine γ-lyase; MPST - 3-mercaptopyruvate sulfurtransferase; CDO - cysteine dioxygenase; RNS - reactive nitrogen species; ROS - reactive oxygen species

The level of sulfane sulfur in human and animal tissues. Data from published experimental works cited in Table 1 were used in Figure 2. The bars correspond to the mean value ± SD (

standard deviation) of sulfane sulfur level expressed in nmoles of SCN− per mg of protein, from three or more determinations in at least three repetitions — The level of sulfane sulfur in human and animal tissues. Data from published experimental works cited in Table 1 were used in Figure 2. The bars correspond to the mean value ± SD ( standard deviation) of sulfane sulfur level expressed in nmoles of SCN− per mg of protein, from three or more determinations in at least three repetitions

The level of sulfane sulfur in human and animal cell cultures. Data from published experimental works cited in Table 2 were used in Figure 3. The bars correspond to the mean value ± SD (

standard deviation) of sulfane sulfur level in a particular cell culture, expressed in nmoles of SCN− per mg of protein, from at least three independent experiments, with three or more determinations in each — The level of sulfane sulfur in human and animal cell cultures. Data from published experimental works cited in Table 2 were used in Figure 3. The bars correspond to the mean value ± SD ( standard deviation) of sulfane sulfur level in a particular cell culture, expressed in nmoles of SCN− per mg of protein, from at least three independent experiments, with three or more determinations in each

The level of sulfane sulfur in Rana ridibunda tissues in the fall and spring seasons. Data from published experimental works [53, 54] were used in Figure 4. The bars correspond to the mean value ± SD (

standard deviation) of sulfane sulfur level expressed in nmoles of SCN− per mg of protein, from three or more determinations in at least three repetitions — The level of sulfane sulfur in Rana ridibunda tissues in the fall and spring seasons. Data from published experimental works [53, 54] were used in Figure 4. The bars correspond to the mean value ± SD ( standard deviation) of sulfane sulfur level expressed in nmoles of SCN− per mg of protein, from three or more determinations in at least three repetitions

The level of sulfane sulfur in selected tissues of male and female mice. Data from published experimental work [51] were used in Figure 5. The bars correspond to the mean value ± SD (

standard deviation) of sulfane sulfur level expressed in nmoles of SCN− per mg of protein, from three or more determinations in at least three repetitions — The level of sulfane sulfur in selected tissues of male and female mice. Data from published experimental work [51] were used in Figure 5. The bars correspond to the mean value ± SD ( standard deviation) of sulfane sulfur level expressed in nmoles of SCN− per mg of protein, from three or more determinations in at least three repetitions

The list of human and animal tissues in which the level of sulfane sulfur was determined by the method of Wood_ The given tissue and the reference referring to it are marked with the same number of asterisks

Tissues	References
Human Liver, Brain	[44]
Rat Liver^, Heart and Kidney_*	^[45], ^[46], ^**[47]
Guinea pig Liver	[45]
Mouse Liver^,^*, Kidney^,^**, Heart^, Spleen^, Brain stem and cortex^*	^[48], ^[49], ^[50], ^**[51]
Rana temporaria Liver, Kidney, Gonads, Brain, Heart	[52]
Rana ridibunda Liver, Kidney, Brain, Heart, Testes, Skeletal muscles	[53], [54], [55]

The level of sulfane sulfur in various frog tissues exposed to heavy metal ions in water — based on results published in [55]

SULFANE SULFUR % of control
	Pb²⁺	Cd²⁺	Hg²⁺
Brain	151.1	137.3	117.3
Liver	91.5	86.8	94.7
Kidney	133.2	109.5	91.7
Heart	Not changed	Not changed	Not changed
Skeletal muscle	Not changed	Not changed	120.3
Testes	Not changed	88.5	114.6

Human and animal cells in which the level of sulfane sulfur was determined by the method of Wood

Cells	References

Mouse astrocytes	[56]

Mouse cell lines	[57]
MNuMG 4T1 MEF B16-F1 WT Naglu^−/−	[58]
J774A.1	[59]

Rat cell line
H9c2	[57]

Human cell lines
Caco-2 HEK MG-63	[57]
U87MG SHSY5Y	[60]
MCF-7	[61]

DOI: https://doi.org/10.2478/ahem-2025-0021 | Journal eISSN: 1732-2693

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Language: English

Page range: 185 - 194

Submitted on: Jun 9, 2025

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Accepted on: Oct 30, 2025

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Published on: Dec 25, 2025

Published by: Hirszfeld Institute of Immunology and Experimental Therapy

In partnership with: Paradigm Publishing Services

Publication frequency: 1 issue per year

Keywords:

sulfane sulfur,

hydrogen sulfide,

sulfurtransferases,

cold cyanolysis

Related subjects:

Molecular biology,

Microbiology and virology,

Basic medical science,

© 2025 Halina Jurkowska, Leszek Rydz, Marta Kaczor-Kamińska, Maria Wróbel, published by Hirszfeld Institute of Immunology and Experimental Therapy
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

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