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DNA and BSA-Binding Studies of Dinuclear Palladium(II) Complexes with 1,5-Naphtiridine Bridging Ligands Cover

DNA and BSA-Binding Studies of Dinuclear Palladium(II) Complexes with 1,5-Naphtiridine Bridging Ligands

EABR. Experimental and Applied Biomedical Research
Volume 25 (2024): Issue 2 (June 2024)
By: Snezana Rajkovic,  Andjela A. Franich,  Vojislav Cupurdija and  Marija D. Zivkovic  
Open Access
|Jun 2021

Figures & Tables

Figure 1.

Structural formulas of 1,5-naphthyridine (1,5-nphe), ethylenediamine (en) and 1,3-propylenediamine (1,3-pd), and dinuclear palladium(II) complexes Pd1 and Pd2.
Structural formulas of 1,5-naphthyridine (1,5-nphe), ethylenediamine (en) and 1,3-propylenediamine (1,3-pd), and dinuclear palladium(II) complexes Pd1 and Pd2.

Figure 2.

1H and 13C NMR spectra of [{Pd(en)Cl}2(μ-1,5-nphe)](NO3)2 (Pd1) and [{Pd(1,3-pd)Cl}2(μ-1,5-nphe)](NO3)2 (Pd2) complexes.
1H and 13C NMR spectra of [{Pd(en)Cl}2(μ-1,5-nphe)](NO3)2 (Pd1) and [{Pd(1,3-pd)Cl}2(μ-1,5-nphe)](NO3)2 (Pd2) complexes.

Figure 3.

UV-Vis spectra of dinuclear [{Pd(en)Cl}2 (μ-1,5-nphe)]2+ and [{Pd(1,3-pd)Cl}2(μ-1,5-nphe)]2+ complexes and free 1,5-nphe ligand.
UV-Vis spectra of dinuclear [{Pd(en)Cl}2 (μ-1,5-nphe)]2+ and [{Pd(1,3-pd)Cl}2(μ-1,5-nphe)]2+ complexes and free 1,5-nphe ligand.

Figure 4.

IR spectra of Pd1 and Pd2 complexes, KBr, 4000-450 cm−1.
IR spectra of Pd1 and Pd2 complexes, KBr, 4000-450 cm−1.

Figure 5.

Absorption spectra of [{Pd(en)Cl}2(μ-1,5-nphe)]2+ and [{Pd(1,3-pd)Cl}2(μ-1,5-nphe)]2+ complexes in 0.01 M phosphate buffer before and after addition of CT-DNA. [Pd(II) complex] = 4.51 · 10−5 M, [DNA] = (0 – 2.00) · 10−4 M.
Absorption spectra of [{Pd(en)Cl}2(μ-1,5-nphe)]2+ and [{Pd(1,3-pd)Cl}2(μ-1,5-nphe)]2+ complexes in 0.01 M phosphate buffer before and after addition of CT-DNA. [Pd(II) complex] = 4.51 · 10−5 M, [DNA] = (0 – 2.00) · 10−4 M.

Figure 6.

EtBr/CT-DNA emission spectra in the presence of [{Pd(en)Cl}2(μ-1,5-nphe)]2+ and [{Pd(1,3-pd)Cl}2(μ-1,5-nphe)]2+ complex. [EtBr] = 1.28 · 10−5 M, [DNA] = 1.28 · 10−5 M, [Pd(II) complex] = (0 – 1.28) · 10−5 M. Inserted graph: Dependence of I0/I from concentration [Q].
EtBr/CT-DNA emission spectra in the presence of [{Pd(en)Cl}2(μ-1,5-nphe)]2+ and [{Pd(1,3-pd)Cl}2(μ-1,5-nphe)]2+ complex. [EtBr] = 1.28 · 10−5 M, [DNA] = 1.28 · 10−5 M, [Pd(II) complex] = (0 – 1.28) · 10−5 M. Inserted graph: Dependence of I0/I from concentration [Q].

Figure 7.

BSA emission spectra in the presence of [{Pd(en)Cl}2(μ-1,5-nphe)]2+ and [{Pd(1,3-pd)Cl}2(μ-1,5-nphe)]2+ complexes. [BSA] = 1.60 · 10−6 M, [Pd(II) complex] = (0 – 4.00) · 10−5 M; λex = 295 nm. Inserted graph: Dependence of Io/I from concentration [Q].
BSA emission spectra in the presence of [{Pd(en)Cl}2(μ-1,5-nphe)]2+ and [{Pd(1,3-pd)Cl}2(μ-1,5-nphe)]2+ complexes. [BSA] = 1.60 · 10−6 M, [Pd(II) complex] = (0 – 4.00) · 10−5 M; λex = 295 nm. Inserted graph: Dependence of Io/I from concentration [Q].

Stern-Volmer constant (Ksv), quenching constant (kq), binding constant (Ka) and number of binding sites (n) for interactions between BSA and dinuclear Pd1 and Pd2 complexes_

complexKsv · 105 (M−1)kq · 1012 (M−1s−1)Ka · 109 (M−1)n
[{Pd(en)Cl}2 (μ-1,5-nphe)]2+(2.80 ± 0.03)(28 ± 2)(1.20 ± 0.05)1.8
[{Pd(1,3-pd)Cl}2 (μ-1,5-nphe)]2+(3.90 ± 0.04)(39 ± 1)(1.20 ± 0.02)1.8

Stern-Volmer constant (Ksv), stability constant (Ka) and number of binding sites (n) of the investigated dinuclear palladium(II) complexes with CT-DNA

complexKsv · 105 (M−1)Ka · 105 (M−1)n
[{Pd(en)Cl}2 (μ-1,5-nphe)]2+(4.50 ± 0.04)(8.80 ± 0.02)1.7
[{Pd(1,3-pd)Cl}2 (μ-1,5-nphe)]2+(2.80 ± 0.06)(2.50 ± 0.05)1.1

1H and 13C NMR chemical shifts (δ, ppm) for 1,5-naphthyridine (1,5-nphe), ethylenediamine (en), 1,3-propylenediamine (1,3-pd) and the corresponding dinuclear palladium(II) complexes

ligand/complexNMR shifts
1H13C
H2, H6H4, H8H3, H7alifatic protonsC2, C6C4, C8C3, C7C4a, C8aL
1,5-nphe8.57 dd7.89 d7.48 dd 154139128144
en 2.64 s (CH2) 45 (CH2)
Pd110.21 d9.58 d8.14 m2.76 m (CH2)16014713014350 (CH2)
1,3-pd 1.60 m (H2, CH2) 2.47 m (H1,H3,CH2) 29 (C2, CH2) 39 (C1,C3, H2)
Pd210.17 d9.55 d8.23 m1.85 m (H2,CH2) 2.76 m (H1,H3,CH2)16014613114330 (C2, CH2) 43,44 (C1,C3, CH2)

Internal binding constants (Kb), changes in Gibbs energy (ΔG) and hypochromism (H) of the investigated dinuclear palladium(II) complexes, Pd1 and Pd2, with CT-DNA

complexKb · 104 (M−1)ΔG (kJ/mol)H
[{Pd(en)Cl}2 (μ-1,5-nphe)]2+(7.50 ± 0.05)−28.9322.42
[{Pd(1,3-pd)Cl}2 (μ-1,5-nphe)]2+(4.00 ± 0.03)−27.3125.77
DOI: https://doi.org/10.2478/sjecr-2021-0030 | Journal eISSN: 2956-2090 | Journal ISSN: 2956-0454
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Language: English
Page range: 113 - 126
Submitted on: Jan 22, 2021
|
Accepted on: Feb 24, 2021
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Published on: Jun 22, 2021
Published by: University of Kragujevac, Faculty of Medical Sciences
In partnership with: Paradigm Publishing Services
Publication frequency: 4 issues per year
Keywords:
Dinuclear palladium(II) complexes,
1,5-naphthyridine,
DNA,
bovine serum albumin (BSA)
Related subjects:
Medicine,
Clinical medicine,
Clinical medicine, other

© 2021 Snezana Rajkovic, Andjela A. Franich, Vojislav Cupurdija, Marija D. Zivkovic, published by University of Kragujevac, Faculty of Medical Sciences
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

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