First Report of Bitylenchus hispaniensis, Pratylenchoides alkani, and Helicotylenchus vulgaris in Association with Cultivated and Wild Olives in Crete, Greece and Molecular Identification of Helicotylenchus microlobus and Merlinius brevidens

Emmanuel A. Tzortzakakis; Carolina Cantalapiedra-Navarrete; Maria Kormpi; Maria S. Lazanaki; Pablo Castillo; Antonio Archidona-Yuste

doi:10.21307/jofnem-2018-020

Figures & Tables

Geographic distribution of plant-parasitic nematodes of the genera Bitylenchus, Helicotylenchus, Merlinius and Pratylenchoides in field survey of cultivated and wild olive in Crete, Greece. Numbers of samples for each nematode species are as follows: Bitylenchus hispaniensis (seven samples), Helicotylenchus microlobus (three samples), Helicotylenchus vulgaris (seven samples), Merlinius brevidens (40 samples) and Pratylenchoides alkani (eight samples). 44 sites are presented, as some samples were derived from spatially close (encircled) areas and may therefore not be independent populations.

Sequenced genes pf plant-parasitic nematodes sampled from cultivated and wild olive in Crete and two other hosts in Evia and Thessaly, Greece_

Nematode species	Sample code	Host and Locality	D2-D3	ITS
Bitylenchus hispaniensis	OLI106	Cultivated olive, Episkopi, Crete	MG770479	–
B. hispaniensis	OLI013	Cultivated olive, Roufas, Crete	*	–
B. hispaniensis	OLE011	Wild olive, Trypiti, Crete	*
B. hispaniensis	OLE015	Wild olive, Lentas, Crete	*	–
B. hispaniensis	OLE023	Wild olive, Ag. Ioannis, Crete	*	–
B. hispaniensis	OLE029	Wild olive, Ag. Georgios, Crete	*	–
B. hispaniensis	OLE032	Wild olive, Tsoutsouros, Crete	*	–
Helicotylenchus microlobus	OLI066	Cultivated olive, Vori, Crete	MG770480	MG770406
H. microlobus	OLI002	Cultivated olive, Vori, Crete	*	–
H. microlobus	OLI074	Cultivated olive, Vori, Crete	*	–
H. microlobus	POT017	Potted cultivated olive, Chania, Crete	MG770481	MG770407
H. microlobus	WAL17	Walnut, Evia, Greece	MG770482	MG770408
Helicotylenchus vulgaris	OLI004	Cultivated olive, Petrokefali, Crete	MG770483	–
H. vulgaris	OLI005	Cultivated olive, Petrokefali, Crete	*	–
H. vulgaris	OLI046	Cultivated olive, Gonies, Crete	*	–
H. vulgaris	OLI055	Cultivated olive, Voutes, Crete	*	–
H. vulgaris	OLI071	Cultivated olive, Pentamodi, Crete	*	–
H. vulgaris	OLI129	Cultivated olive, Perama, Crete	*	–
H. vulgaris	OLE026	Wild olive, Agiofarago, Crete	*	–
H. vulgaris	GOBERR	Goji berry, Thessaly, Greece	MG770484	–
Merlinius brevidens ^a	OLI106	Cultivated olive, Episkopi, Crete	MG770485	–
M. brevidens	OLE009	Wild olive, Kefali, Crete	*	–
Pratylenchoides alkani ^b	OLI060	Cultivated olive, Sivas, Crete	MG770486	–
P. alkani	OLI111	Cultivated olive, Amygdali, Crete	*	–
P. alkani	OLE007	Wild olive, Vathy, Crete	*	–
P. alkani	OLE017	Wild olive, Agiofarago, Crete	*	–
P. alkani	OLE018	Wild olive, Agiofarago, Crete	*	–
P. alkani	OLE031	Wild olive, Listis, Crete	*	–
P. alkani	OLE032	Wild olive, Tsoutsouros, Crete	*	–
P. alkani	OLE036	Wild olive, Agiofarago, Crete	*	–

Morphometrics of Bitylenchus hispaniensis (Handoo, et al_, 2014); Helicotylenchus microlobus (Perry et al_, 1959); Helicotylenchus vulgaris (Yuen, 1964); Merlinius brevidens (Allen, 1955; Siddiqi, 1970); and Pratylenchoides alkani (Yüksel, 1977) from olive in Crete, Greecea_

	B. hispaniensis	H. microlobus	H. vulgaris	M. brevidens	P. alkani
	Episkopi	Vori	Petrokefali	Episkopi	Sivas
Characters/ratios^b	Females	Females	Females	Females	Females
n	3	4	3	3	3
L	722 ± 47 (670–760)	652 ± 31 (609–680)	672 ± 35 (634–702)	564 ± 116 (490–698)	907 ± 69.1 (840–978)
a	32.3 ± 0.6 (31.9–33.0)	25.4 ± 1.6 (24.2–27.7)	30.6 ± 0.4 (30.2–31.0)	26.3 ± 3.8 (22.9–30.3)	35.3 ± 0.8 (34.7–36.2)
B	6.3 ± 0.4 (6.1–6.8)	4.5 ± 0.4 (4.1–4.9)	5.1 ± 0.02 (5.1–5.2)	4.4 ± 0.6 (4.0–5.1)	6.7 ± 0.2 (6.4–6.8)
C	14.9 ± 0.6 (14.4–15.5)	43.7 ± 8.4 (35.8–55.4)	48.1 ± 1.1 (46.8–48.8)	14.3 ± 2.4 (12.9–17.0)	16.7 ± 0.2 (16.5–16.9)
c′	3.0 ± 0.2 (2.7–3.2)	1.1 ± 0.2 (0.9–1.4)	1.1 ± 0.0 (1.1–1.1)	3.0 ± 0.1 (2.9–3.2)	3.1 ± 0.2 (2.8–3.2)
V	55.0 ± 1.0 (54.0–56.0)	61.2 ± 1.6 (59.3–63.2)	64.0 ± 2.0 (62.0–66.0)	54.7 ± 3.1 (52.0–58.0)	52.7 ± 1.5 (51.0–54.0)
Stylet length	17.3 ± 0.3 (17.0–17.5)	26.4 ± 1.2 (25.0–28.0)	24.0 ± 1.0 (23.0–25.0)	14.3 ± 1.5 (13.0–16.0)	23.7 ± 0.6 (23.0–24.0)
Pharynx length	114.0 ± 5.3 (110–120)	145.5 ± 7.0 (139–153)	130.7 ± 6.8 (123–136)	127.7 ± 7.2 (123–136)	135.7 ± 11.2 (123–144)
Max. body diam.	22.3 ± 1.2 (21.0–23.0)	25.7 ± 1.5 (24.0–27.0)	22.0 ± 1.0 (21.0–23.0)	21.3 ± 2.1 (19.0–23.0)	25.7 ± 1.5 (24.0–27.0)
Anal body diam.	16.5 ± 2.2 (14.0–18.0)	14.1 ± 1.4 (12.5–16.0)	13.0 ± 1.0 (12.0–14.0)	13.0 ± 1.0 (12.0–14.0)	17.7 ± 0.6 (17.0–18.0)
Tail	48.3 ± 3.1 (45.0–51.0)	15.3 ± 2.4 (12.0–17.0)	14.0 ± 1.0 (13.0–15.0)	39.3 ± 1.5 (38.0–41.0)	54.3 ± 3.5 (51.0–58.0)

First Report of Bitylenchus hispaniensis, Pratylenchoides alkani, and Helicotylenchus vulgaris in Association with Cultivated and Wild Olives in Crete, Greece and Molecular Identification of Helicotylenchus microlobus and Merlinius brevidens

Figures & Tables

Fig. 1

Sequenced genes pf plant-parasitic nematodes sampled from cultivated and wild olive in Crete and two other hosts in Evia and Thessaly, Greece_

Morphometrics of Bitylenchus hispaniensis (Handoo, et al_, 2014); Helicotylenchus microlobus (Perry et al_, 1959); Helicotylenchus vulgaris (Yuen, 1964); Merlinius brevidens (Allen, 1955; Siddiqi, 1970); and Pratylenchoides alkani (Yüksel, 1977) from olive in Crete, Greecea_

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