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Biological characterization of the entomopathogenic nematode, Steinernema innovationi: a South African isolate Cover

Biological characterization of the entomopathogenic nematode, Steinernema innovationi: a South African isolate

Journal of Nematology
Volume 50 (2018): Issue 4 (January 2018)
By: Tshimangadzo Ramakuwela,  Justin Hatting,  Mark D. Laing,  Nicolene Thiebaut and  Selcuk Hazir  
Open Access
|Dec 2018

Figures & Tables

Figure 1

Galleria mellonella larvae inoculated with Steinernema innovationi (50 infective juveniles (IJs)/insect) after 10 d of incubation at different temperatures (10, 15, 20, 25, and 30°C). (A) Mean percentage mortality; (B) Number of days until death; (C) Number of IJ established in G. mellonella larvae. Standard error bars with the same lowercase letter indicate no significant difference.
Galleria mellonella larvae inoculated with Steinernema innovationi (50 infective juveniles (IJs)/insect) after 10 d of incubation at different temperatures (10, 15, 20, 25, and 30°C). (A) Mean percentage mortality; (B) Number of days until death; (C) Number of IJ established in G. mellonella larvae. Standard error bars with the same lowercase letter indicate no significant difference.

Figure 2

Galleria mellonella inoculated with Steinernema innovationi (100 infective juveniles (IJ)/insect) and incubated to study nematode development at five different temperatures (18, 20, 22, 25, and 27°C). (A) Days until emergence; (B) Yield (number of IJ) per gram of G. mellonella. Standard error bars with the same lowercase letter indicate no significant difference.
Galleria mellonella inoculated with Steinernema innovationi (100 infective juveniles (IJ)/insect) and incubated to study nematode development at five different temperatures (18, 20, 22, 25, and 27°C). (A) Days until emergence; (B) Yield (number of IJ) per gram of G. mellonella. Standard error bars with the same lowercase letter indicate no significant difference.

Figure 3

Mean number of infective juveniles (IJs) of Steinernema innovationi established in Galleria mellonella in sand columns at different depths after 24, 48, and 72 hr post inoculation with 100 IJs on the top surface of the sand column. Standard error bars with the same lowercase letter indicate no significant difference.
Mean number of infective juveniles (IJs) of Steinernema innovationi established in Galleria mellonella in sand columns at different depths after 24, 48, and 72 hr post inoculation with 100 IJs on the top surface of the sand column. Standard error bars with the same lowercase letter indicate no significant difference.

Figure 4

Mean mortality and confirmation of nematode development in different insect hosts post inoculation with 50 or 500 infective juveniles/host of Steinernema innovationi. Mortality was corrected using Schneider-Orelli’s formula (Püntener, 1981). *7 days incubation time.
Mean mortality and confirmation of nematode development in different insect hosts post inoculation with 50 or 500 infective juveniles/host of Steinernema innovationi. Mortality was corrected using Schneider-Orelli’s formula (Püntener, 1981). *7 days incubation time.

Figure 5

Comparison of mean mortality of larvae vs pupae per insect host following infection with 50 or 500 infective juveniles/host of Steinernema innovationi. Mortality was corrected using Schneider-Orelli’s formula (Püntener, 1981).
Comparison of mean mortality of larvae vs pupae per insect host following infection with 50 or 500 infective juveniles/host of Steinernema innovationi. Mortality was corrected using Schneider-Orelli’s formula (Püntener, 1981).
DOI: https://doi.org/10.21307/jofnem-2018-049 | Journal eISSN: 2640-396X | Journal ISSN: 0022-300X
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Language: English
Page range: 1 - 10
Published on: Dec 3, 2018
Published by: Society of Nematologists, Inc.
In partnership with: Paradigm Publishing Services
Publication frequency: 1 issue per year
Keywords:
Entomopathogenic nematode,
Foraging behavior,
Host range,
nematode reproduction,
Optimum temperature,
Virulence.
Related subjects:
Life sciences,
Life sciences, other

© 2018 Tshimangadzo Ramakuwela, Justin Hatting, Mark D. Laing, Nicolene Thiebaut, Selcuk Hazir, published by Society of Nematologists, Inc.
This work is licensed under the Creative Commons Attribution 4.0 License.

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